Multiplexed enrichment and genomic profiling of peripheral blood cells reveal subset-specific immune signatures
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eaau9223.full.pdf
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3.12 MB
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Checksum (MD5)
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Author(s)
Vickers, Dwayne
Billman, Kianna
Eisenhaure, Thomas
Hoover, Paul
Browne, Edward P.
Rao, Deepak A.
Hacohen, Nir
Reyes, Miguel
Blainey, Paul C
Date Issued
January 2019
Journal
Science Advances
Publisher
American Association for the Advancement of Science (AAAS)
Citation
Reyes, Miguel et al. “Multiplexed Enrichment and Genomic Profiling of Peripheral Blood Cells Reveal Subset-Specific Immune Signatures.” Science Advances 5, 1 (January 2019): eaau9223 © 2019 The Authors
Version
Final published version
Abstract
Specialized immune cell subsets are involved in autoimmune disease, cancer immunity, and infectious disease through a diverse range of functions mediated by overlapping pathways and signals. However, subset-specific responses may not be detectable in analyses of whole blood samples, and no efficient approach for profiling cell subsets at high throughput from small samples is available. We present a low-input microfluidic system for sorting immune cells into subsets and profiling their gene expression. We validate the system’s technical performance against standard subset isolation and library construction protocols and demonstrate the importance of subset-specific profiling through in vitro stimulation experiments. We show the ability of this integrated platform to identify subset-specific disease signatures by profiling four immune cell subsets in blood from patients with systemic lupus erythematosus (SLE) and matched control subjects. The platform has the potential to make multiplexed subset-specific analysis routine in many research laboratories and clinical settings.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Terms of Use
Creative Commons Attribution NonCommercial License 4.0
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DOI of Published Version
https://doi.org/10.1126/sciadv.aau9223
