Osteoconductive protamine-based polyelectrolyte multilayer functionalized surfaces
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Hammond_Osteoconductive protamine-based.pdf
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Author(s)
Samuel, Raymond E.
Paik, Daniel H.
Fang, Jean C.
Schmidt, Daniel J.
Shukla, A.
Wang, Mary
Hammond, Paula T
Date Issued
July 2011
Journal
Biomaterials
Publisher
Elsevier
Citation
Samuel, Raymond E., Anita Shukla, Daniel H. Paik, Mary X. Wang, Jean C. Fang, Daniel J. Schmidt, and Paula T. Hammond. “Osteoconductive Protamine-Based Polyelectrolyte Multilayer Functionalized Surfaces.” Biomaterials 32, no. 30 (October 2011): 7491–7502.
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Author's final manuscript
Abstract
The integration of orthopedic implants with host bone presents a major challenge in joint arthroplasty, spinal fusion and tumor reconstruction. The cellular microenvironment can be programmed via implant surface functionalization allowing direct modulation of osteoblast adhesion, proliferation, and differentiation at the implant–bone interface. The development of layer-by-layer assembled polyelectrolyte multilayer (PEM) architectures has greatly expanded our ability to fabricate intricate nanometer to micron scale thin film coatings that conform to complex implant geometries. The in vivo therapeutic efficacy of thin PEM implant coatings for numerous biomedical applications has previously been reported. We have fabricated protamine-based PEM thin films that support the long-term proliferation and differentiation of pre-osteoblast cells on non-cross-linked film-coated surfaces. These hydrophilic PEM functionalized surfaces with nanometer-scale roughness facilitated increased deposition of calcified matrix by osteoblasts in vitro, and thus offer the potential to enhance implant integration with host bone. The coatings can make an immediate impact in the osteogenic culture of stem cells and assessment of the osteogenic potential of new therapeutic factors.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Koch Institute for Integrative Cancer Research at MIT
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Creative Commons Attribution-Noncommercial-NoDerivatives
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DOI of Published Version
https://doi.org/10.1016/j.biomaterials.2011.06.032
