Large-scale voltage imaging in behaving mice using targeted illumination
Name
1-s2.0-S2589004221012323-main.pdf
Description
Published version
Size
7.36 MB
Format
Adobe PDF
Checksum (MD5)
0eaca7598d2c539657d3fe31a1d89417
Author(s)
Xiao, Sheng
Lowet, Eric
Gritton, Howard J
Fabris, Pierre
Wang, Yangyang
Sherman, Jack
Mount, Rebecca A
Tseng, Hua-an
Man, Heng-Ye
Straub, Christoph
Date Issued
2021
Journal
iScience
Publisher
Elsevier BV
Citation
Xiao, Sheng, Lowet, Eric, Gritton, Howard J, Fabris, Pierre, Wang, Yangyang et al. 2021. "Large-scale voltage imaging in behaving mice using targeted illumination." iScience, 24 (11).
Version
Final published version
Abstract
Recent improvements in genetically encoded voltage indicators enabled optical imaging of action potentials and subthreshold transmembrane voltage in vivo. To perform high-speed voltage imaging of many neurons simultaneously over a large anatomical area, widefield microscopy remains an essential tool. However, the lack of optical sectioning makes widefield microscopy prone to background cross-contamination. We implemented a digital-micromirror-device-based targeted illumination strategy to restrict illumination to the cells of interest and quantified the resulting improvement both theoretically and experimentally with SomArchon expressing neurons. We found that targeted illumination increased SomArchon signal contrast, decreased photobleaching, and reduced background cross-contamination. With the use of a high-speed, large-area sCMOS camera, we routinely imaged tens of spiking neurons simultaneously over minutes in behaving mice. Thus, the targeted illumination strategy described here offers a simple solution for widefield voltage imaging of many neurons over a large field of view in behaving animals.
MIT Department
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/J.ISCI.2021.103263
