Fatty Acid Uptake and Lipid Storage Induced by HIF-1α Contribute to Cell Growth and Survival after Hypoxia-Reoxygenation
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Bensaad-2014-Fatty acid uptake an.pdf
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Author(s)
Bensaad, Karim
Favaro, Elena
Lewis, Caroline A.
Peck, Barrie
Lord, Simon
Collins, Jennifer M.
Pinnick, Katherine E.
Wigfield, Simon
Buffa, Francesca M.
Li, Ji-Liang
Date Issued
October 2014
Journal
Cell Reports
Citation
Bensaad, Karim, Elena Favaro, Caroline A. Lewis, Barrie Peck, Simon Lord, Jennifer M. Collins, Katherine E. Pinnick, et al. “Fatty Acid Uptake and Lipid Storage Induced by HIF-1α Contribute to Cell Growth and Survival after Hypoxia-Reoxygenation.” Cell Reports 9, no. 1 (October 2014): 349–65.
Version
Final published version
Abstract
An in vivo model of antiangiogenic therapy allowed us to identify genes upregulated by bevacizumab treatment, including Fatty Acid Binding Protein 3 (FABP3) and FABP7, both of which are involved in fatty acid uptake. In vitro, both were induced by hypoxia in a hypoxia-inducible factor-1α (HIF-1α)-dependent manner. There was a significant lipid droplet (LD) accumulation in hypoxia that was time and O[subscript 2] concentration dependent. Knockdown of endogenous expression of FABP3, FABP7, or Adipophilin (an essential LD structural component) significantly impaired LD formation under hypoxia. We showed that LD accumulation is due to FABP3/7-dependent fatty acid uptake while de novo fatty acid synthesis is repressed in hypoxia. We also showed that ATP production occurs via β-oxidation or glycogen degradation in a cell-type-dependent manner in hypoxia-reoxygenation. Finally, inhibition of lipid storage reduced protection against reactive oxygen species toxicity, decreased the survival of cells subjected to hypoxia-reoxygenation in vitro, and strongly impaired tumorigenesis in vivo.
MIT Department
Koch Institute for Integrative Cancer Research at MIT
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DOI of Published Version
https://doi.org/10.1016/j.celrep.2014.08.056
