Reply to Grace: Role of cholinergic neurons in rapid eye movement (REM) sleep control
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PNAS-2015-Van Dort-E3756.pdf
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Author(s)
Van Dort, Christa Joy
Brown, Emery Neal
Date Issued
June 2015
Journal
Proceedings of the National Academy of Sciences
Publisher
Proceedings of the National Academy of Sciences
Citation
Van Dort, Christa J., and Brown, Emery N. “Reply to Grace: Role of Cholinergic Neurons in Rapid Eye Movement (REM) Sleep Control.” Proceedings of the National Academy of Sciences 112, 29 (June 2015): E3756–E3756 © 2015 National Academy Sciences
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Final published version
Abstract
We thank Grace (1) for the opportunity to discuss the role of cholinergic neurons in rapid eye movement (REM) sleep further. Grace suggests that optogenetic activation of a population of neurons does not necessarily demonstrate their role in the endogenous system when interrogating complex neural circuitry. We agree that we do not prove necessity of cholinergic neurons in REM sleep generation, as we point out in our discussion, “Future studies that selectively inhibit cholinergic neurons in the PPT [pedunculopontine tegmentum] and LDT [laterodorsal tegmentum] of nonhypercholinergic mice are needed to determine if cholinergic neurons are necessary for REM sleep generation” (2). However, in our report we do demonstrate the sufficiency of PPT/LDT neurons to influence REM sleep initiation but not influence REM sleep duration, thus distinguishing the role of cholinergic neurons on these properties of REM sleep. In addition, activation of cholinergic PPT neurons during non-REM sleep induced REM sleep versus wakefulness. Our data are consistent with the role of cholinergic neurons in generating an activated brain state and many studies pointing to the role of cholinergic neurons in REM sleep regulation (reviewed in ref. 3).
MIT Department
Institute for Medical Engineering and Science
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Picower Institute for Learning and Memory
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DOI of Published Version
https://doi.org/10.1073/PNAS.1506854112
