In vitro and ex vivo strategies for intracellular delivery
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in vitro and ex vivo.pdf
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629.11 KB
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Author(s)
Sahay, Gaurav
Stewart, Martin P
Sharei, Armon Reza
Ding, Xiaoyun Sean
Langer, Robert S
Jensen, Klavs F
Date Issued
October 2016
Journal
Nature
Publisher
Nature Publishing Group
Citation
Stewart, Martin P.; Sharei, Armon; Ding, Xiaoyun et al. “In Vitro and Ex Vivo Strategies for Intracellular Delivery.” Nature 538, 7624 (October 2016): 183–192 © 2016 Macmillan Publishers Limited, part of Springer Nature
Version
Author's final manuscript
Abstract
Intracellular delivery of materials has become a critical component of genome-editing approaches, ex vivo cell-based therapies, and a diversity of fundamental research applications. Limitations of current technologies motivate development of next-generation systems that can deliver a broad variety of cargo to diverse cell types. Here we review in vitro and ex vivo intracellular delivery approaches with a focus on mechanisms, challenges and opportunities. In particular, we emphasize membrane-disruption-based delivery methods and the transformative role of nanotechnology, microfluidics and laboratory-on-chip technology in advancing the field.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1038/nature19764
