Culturing human intestinal stem cells for regenerative applications in the treatment of inflammatory bowel disease
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558.full.pdf
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3.83 MB
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Author(s)
Holmberg, Fredrik EO
Seidelin, Jakob B
Tong, Zhixiang
Li, Yuan
Nielsen, Ole H
Yin, Xiaolei
Mead, Benjamin Elliott
Karp, Jeffrey Michael
Date Issued
March 2017
Journal
EMBO Molecular Medicine
Publisher
Wiley Blackwell
Citation
Holmberg, Fredrik EO et al. “Culturing Human Intestinal Stem Cells for Regenerative Applications in the Treatment of Inflammatory Bowel Disease.” EMBO Molecular Medicine 9, 5 (March 2017): 558–570 © 2017 The Authors
Version
Final published version
Abstract
Both the incidence and prevalence of inflammatory bowel disease (IBD) is increasing globally; in the industrialized world up to 0.5% of the population are affected and around 4.2 million individuals suffer from IBD in Europe and North America combined. Successful engraftment in experimental colitis models suggests that intestinal stem cell transplantation could constitute a novel treatment strategy to re-establish mucosal barrier function in patients with severe disease. Intestinal stem cells can be grown in vitro in organoid structures, though only a fraction of the cells contained are stem cells with regenerative capabilities. Hence, techniques to enrich stem cell populations are being pursued through the development of multiple two-dimensional and three-dimensional culture protocols, as well as co-culture techniques and multiple growth medium compositions. Moreover, research in support matrices allowing for efficient clinical application is in progress. In vitro culture is accomplished by modulating the signaling pathways fundamental for the stem cell niche with a suitable culture matrix to provide additional contact-dependent stimuli and structural support. The aim of this review was to discuss medium compositions and support matrices for optimal intestinal stem cell culture, as well as potential modifications to advance clinical use in IBD. Keywords: inflammatory bowel disease; intestinal stem cells; organoids; regenerative medicine; support matrix
MIT Department
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Attribution 4.0 International (CC BY 4.0)
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DOI of Published Version
https://doi.org/10.15252/emmm.201607260
