Design, construction and characterization of a set of insulated bacterial promoters
Name
Davis-2011-Design, construction and characterization of a set.pdf
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519.79 KB
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Adobe PDF
Checksum (MD5)
3a906dae43ca06dd051d334c33fcffb8
Author(s)
Davis, Joseph Harry
Rubin, Adam J.
Sauer, Robert T.
Date Issued
September 2010
Journal
Nucleic Acids Research
Publisher
Oxford University Press
Citation
Davis, J. H., A. J. Rubin, and R. T. Sauer. “Design, Construction and Characterization of a Set of Insulated Bacterial Promoters.” Nucleic Acids Research 39.3 (2010): 1131–1141. Web.
Version
Final published version
Abstract
We have generated a series of variable-strength, constitutive, bacterial promoters that act predictably in different sequence contexts, span two orders of magnitude in strength and contain convenient sites for cloning and the introduction of downstream open-reading frames. Importantly, their design insulates these promoters from the stimulatory or repressive effects of many 5′- or 3′-sequence elements. We show that different promoters from our library produce constant relative levels of two different proteins in multiple genetic contexts. This set of promoters should be a useful resource for the synthetic-biology community.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution Non-Commercial
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DOI of Published Version
https://doi.org/10.1093/nar/gkq810
