Fabrication and function of microfluidic devices for monitoring of in-vitro fertilization processes
Name
212409168-MIT.pdf
Description
Full printable version
Size
2.34 MB
Format
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Checksum (MD5)
c8632d919f4e7a6181474edc76e16a9b
Author(s)
Xu, Jin (Jin C.)
Advisor(s)
Todd Thorsen.
Date Issued
2007
Publisher
Massachusetts Institute of Technology
Abstract
The process of assistive reproduction is often a headache and heartache for those who choose to go through it. The field currently relies heavily on morphological characteristics to determine embryo health and development success, a highly unreliable method. While they appear healthy at implantation, many embryos, in reality, have poor development potential and fail to survive within the womb. Therefore, to offset the high chances of miscarriage, multiple eggs are implanted in the uterus. This has occasionally lead to multi-fetal pregnancies, which have a higher maternal mortality risk, and, in general, is more physically demanding. This thesis researches a microfluidic device that aids in the crucial stages of in vitro- fertilization. The device allows for a fertilized egg to be cultured within, and provides the ability to carefully monitor its health through a series of metabolic assays, a better indication of embryo health. This microfluidic embryo health monitoring device is comprised of two layers of channel networks. It works through passing fluids along flow channels that are driven by control channels. The control layer, when pressurized with gas, operates as valves and peristaltic pumps along the flow layer to pump and transport fluids through the flow channels. As embryonic fluids are passed through the channels, the status of the fertilized egg can be monitored with metabolic assays taken of the embryo at various detection sites.
Description
Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2007.
Includes bibliographical references (leaf 36).
Subjects
Mechanical Engineering.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
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