Silicone cryogel skeletons enhance the survival and mechanical integrity of hydrogel-encapsulated cell therapies
Name
sciadv.adk5949.pdf
Description
Published version
Size
17.45 MB
Format
Adobe PDF
Checksum (MD5)
31036be4e7d171087576a579902513ee
Author(s)
Jeang, William J
Bochenek, Matthew A
Bose, Suman
Zhao, Yichao
Wong, Bryan M
Yang, Jiawei
Jiang, Alexis L
Langer, Robert
Anderson, Daniel G
Date Issued
April 5, 2024
Journal
Science Advances
Publisher
American Association for the Advancement of Science
Citation
William J. Jeang et al. ,Silicone cryogel skeletons enhance the survival and mechanical integrity of hydrogel-encapsulated cell therapies.Sci. Adv.10,eadk5949(2024).
Version
Final published version
Abstract
The transplantation of engineered cells that secrete therapeutic proteins presents a promising method for addressing a range of chronic diseases. However, hydrogels used to encase and protect non-autologous cells from immune rejection often suffer from poor mechanical properties, insufficient oxygenation, and fibrotic encapsulation. Here, we introduce a composite encapsulation system comprising an oxygen-permeable silicone cryogel skeleton, a hydrogel matrix, and a fibrosis-resistant polymer coating. Cryogel skeletons enhance the fracture toughness of conventional alginate hydrogels by 23-fold and oxygen diffusion by 2.8-fold, effectively mitigating both implant fracture and hypoxia of encapsulated cells. Composite implants containing xenogeneic cells engineered to secrete erythropoietin significantly outperform unsupported alginate implants in therapeutic delivery over 8 weeks in immunocompetent mice. By improving mechanical resiliency and sustaining denser cell populations, silicone cryogel skeletons enable more durable and miniaturized therapeutic implants.
MIT Department
Massachusetts Institute of Technology. Department of Materials Science and Engineering
Koch Institute for Integrative Cancer Research at MIT
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Harvard University--MIT Division of Health Sciences and Technology
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Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.1126/sciadv.adk5949
