Spectrum resolved fluorescence imaging in multi-focal volume holographic microscopy
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Kamm_Spectrum resolved.pdf
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1.19 MB
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Author(s)
Luo, Yuan
Zervantonakis, Ioannis
Oh, Se Baek
Kamm, Roger Dale
Barbastathis, George
Date Issued
2011
Journal
Proceedings of SPIE--the International Society for Optical Engineering v.7891
Publisher
SPIE
Citation
Luo, Yuan et al. “Spectrum resolved fluorescence imaging in multi-focal volume holographic microscopy.” Proceedings of SPIE--the International Society for Optical Engineering v.7891, 2011. 78910C–78910C–5. © SPIE 2011
Version
Final published version
Abstract
A real-time three dimensional (3D) fluorescence imaging system incorporating wavelength-coded and multiplexed holographic gratings is presented. Holographic gratings formed in thick Phenanthrenquinone- (PQ-) Doped Poly (methyl methacrylate) (PMMA) have narrowband spectral-spatial transmittance filtering properties to generate wavelengthspectrum selective multi-focal planes within a biological object. We demonstrate the imaging modality to obtain laserinduced fluorescent tissue structures from different depths at the excitation wavelength of 355nm.
MIT Department
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Massachusetts Institute of Technology. Department of Mechanical Engineering
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1117/12.874570
