In vitro synthesis of gene-length single-stranded DNA
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Author(s)
Veneziano, Remi
Shepherd, Tyson R
Ratanalert, Sakul
Bellou, Leila
Tao, Chaoqun
Bathe, Mark
Date Issued
April 2018
Journal
Scientific Reports
Publisher
Nature Publishing Group
Citation
Veneziano, Rémi et al. “In Vitro Synthesis of Gene-Length Single-Stranded DNA.” Scientific Reports 8, 1 (April 2018): 6548 © 2018 The Author(s).
Version
Final published version
Abstract
Single-stranded DNA (ssDNA) increases the likelihood of homology directed repair with reduced cellular toxicity. However, ssDNA synthesis strategies are limited by the maximum length attainable, ranging from a few hundred nucleotides for chemical synthesis to a few thousand nucleotides for enzymatic synthesis, as well as limited control over nucleotide composition. Here, we apply purely enzymatic synthesis to generate ssDNA greater than 15 kilobases (kb) using asymmetric PCR, and illustrate the incorporation of diverse modified nucleotides for therapeutic and theranostic applications.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Chemical Engineering
Terms of Use
Creative Commons Attribution 4.0 International License
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DOI of Published Version
https://doi.org/10.1038/s41598-018-24677-5
