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Intravital imaging of mouse embryos
Name
nihms-1638993.pdf
Description
Accepted version
Size
1.48 MB
Format
Adobe PDF
Checksum (MD5)
ae89cc01ebc8b320911949ea8278fc98
Author(s)
Huang, Qiang
Cohen, Malkiel A
Alsina, Fernando C
Devlin, Garth
Garrett, Aliesha
McKey, Jennifer
Havlik, Patrick
Rakhilin, Nikolai
Wang, Ergang
Xiang, Kun
Date Issued
2020
Journal
Science
Publisher
American Association for the Advancement of Science (AAAS)
Version
Author's final manuscript
Abstract
© 2020 The Authors. Embryonic development is a complex process that is unamenable to direct observation. In this study, we implanted a window to the mouse uterus to visualize the developing embryo from embryonic day 9.5 to birth. This removable intravital window allowed manipulation and high-resolution imaging. In live mouse embryos, we observed transient neurotransmission and early vascularization of neural crest cell (NCC)-derived perivascular cells in the brain, autophagy in the retina, viral gene delivery, and chemical diffusion through the placenta. We combined the imaging window with in utero electroporation to label and track cell division and movement within embryos and observed that clusters of mouse NCC-derived cells expanded in interspecies chimeras, whereas adjacent human donor NCC-derived cells shrank. This technique can be combined with various tissue manipulation and microscopy methods to study the processes of development at unprecedented spatiotemporal resolution.
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
10.1126/SCIENCE.ABA0210
