Measuring single cell mass, volume, and density with dual suspended microchannel resonators
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Author(s)
Shen, Wenjiang
Grover, William H.
Hecht, Vivian Chaya
Payer, Kristofor Robert
Bryan, Andrea Kristine
Manalis, Scott R
Date Issued
December 2013
Journal
Lab on a Chip
Publisher
Royal Society of Chemistry
Citation
Bryan, Andrea K., Vivian C. Hecht, Wenjiang Shen, Kristofor Payer, William H. Grover, and Scott R. Manalis. “Measuring Single Cell Mass, Volume, and Density with Dual Suspended Microchannel Resonators.” Lab Chip 14, no. 3 (2014): 569–576.
Version
Author's final manuscript
Abstract
Cell size, measured as either volume or mass, is a fundamental indicator of cell state. Far more tightly regulated than size is density, the ratio between mass and volume, which can be used to distinguish between cell populations even when volume and mass appear to remain constant. Here we expand upon a previous method for measuring cell density involving a suspended microchannel resonator (SMR). We introduce a new device, the dual SMR, as a high-precision instrument for measuring single-cell mass, volume, and density using two resonators connected by a serpentine fluidic channel. The dual SMR designs considered herein demonstrate the critical role of channel geometry in ensuring proper mixing and damping of pressure fluctuations in microfluidic systems designed for precision measurement. We use the dual SMR to compare the physical properties of two well-known cancer cell lines: human lung cancer cell H1650 and mouse lymphoblastic leukemia cell line L1210.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Microsystems Technology Laboratories
Koch Institute for Integrative Cancer Research at MIT
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1039/c3lc51022k
