Genomically encoded analog memory with precise in vivo DNA writing in living cell populations
Name
Lu_Genomically encoded.pdf
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1.49 MB
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Author(s)
Farzadfard, Fahim
Lu, Timothy K
Date Issued
November 2014
Journal
Science
Publisher
American Association for the Advancement of Science (AAAS)
Citation
Farzadfard, F., and T. K. Lu. “Genomically Encoded Analog Memory with Precise in Vivo DNA Writing in Living Cell Populations.” Science 346, no. 6211 (November 13, 2014): 1256272–1256272.
Version
Author's final manuscript
Abstract
Cellular memory is crucial to many natural biological processes and sophisticated synthetic biology applications. Existing cellular memories rely on epigenetic switches or recombinases, which are limited in scalability and recording capacity. In this work, we use the DNA of living cell populations as genomic “tape recorders” for the analog and distributed recording of long-term event histories. We describe a platform for generating single-stranded DNA (ssDNA) in vivo in response to arbitrary transcriptional signals. When coexpressed with a recombinase, these intracellularly expressed ssDNAs target specific genomic DNA addresses, resulting in precise mutations that accumulate in cell populations as a function of the magnitude and duration of the inputs. This platform could enable long-term cellular recorders for environmental and biomedical applications, biological state machines, and enhanced genome engineering strategies.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Massachusetts Institute of Technology. Microbiology Graduate Program
Massachusetts Institute of Technology. Research Laboratory of Electronics
Massachusetts Institute of Technology. Synthetic Biology Center
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1126/science.1256272
