Genome scanning : an AFM-based DNA sequencing technique
Name
69017720-MIT.pdf
Description
Full printable version
Size
15.37 MB
Format
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Checksum (MD5)
c9e9d5f9387d1dc1bef82b8b6e5a94e4
Author(s)
Elmouelhi, Ahmed (Ahmed M.), 1979-
Advisor(s)
Kamal Youcef-Toumi.
Alternative Title
Atomic force microscopy-based deoxyribonucleic acid sequencing technique
Date Issued
2003
Publisher
Massachusetts Institute of Technology
Abstract
Genome Scanning is a powerful new technique for DNA sequencing. The method presented in this thesis uses an atomic force microscope with a functionalized cantilever tip to sequence single stranded DNA immobilized to a mica surface. The functionalized cantilever tip hybridizes with only one base type (A, C, T, or G) and results in distinct peaks in the AFM-produced image. Genome Scanning has been successful at identifying 40 base strands of synthesized DNA and has been shown to detect a particular base type on 48 kilobase strands of lambda DNA. Currently, Genome Scanning is only accurate to 3-26 bases at a time, however, it can achieve a sequencing speed of 6000 bases/sec. In other words, Genome Scanning can be used to sequence the 3 billion bases of the human genome in 5.78 days.
Description
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2003.
Includes bibliographical references (p. 157-160).
Subjects
Mechanical Engineering.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
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