The cellular environment shapes the nuclear pore complex architecture
Name
s41586-021-03985-3.pdf
Description
Published version
Size
10.03 MB
Format
Adobe PDF
Checksum (MD5)
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Author(s)
Schuller, Anthony P
Wojtynek, Matthias
Mankus, David
Tatli, Meltem
Kronenberg-Tenga, Rafael
Regmi, Saroj G
Dip, Phat V
Lytton-Jean, Abigail KR
Brignole, Edward J
Dasso, Mary
Date Issued
2021
Journal
Nature
Publisher
Springer Science and Business Media LLC
Citation
Schuller, Anthony P, Wojtynek, Matthias, Mankus, David, Tatli, Meltem, Kronenberg-Tenga, Rafael et al. 2021. "The cellular environment shapes the nuclear pore complex architecture." Nature, 598 (7882).
Version
Final published version
Abstract
AbstractNuclear pore complexes (NPCs) create large conduits for cargo transport between the nucleus and cytoplasm across the nuclear envelope (NE)1–3. These multi-megadalton structures are composed of about thirty different nucleoporins that are distributed in three main substructures (the inner, cytoplasmic and nucleoplasmic rings) around the central transport channel4–6. Here we use cryo-electron tomography on DLD-1 cells that were prepared using cryo-focused-ion-beam milling to generate a structural model for the human NPC in its native environment. We show that—compared with previous human NPC models obtained from purified NEs—the inner ring in our model is substantially wider; the volume of the central channel is increased by 75% and the nucleoplasmic and cytoplasmic rings are reorganized. Moreover, the NPC membrane exhibits asymmetry around the inner-ring complex. Using targeted degradation of Nup96, a scaffold nucleoporin of the cytoplasmic and nucleoplasmic rings, we observe the interdependence of each ring in modulating the central channel and maintaining membrane asymmetry. Our findings highlight the inherent flexibility of the NPC and suggest that the cellular environment has a considerable influence on NPC dimensions and architecture.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution 4.0 International license
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DOI of Published Version
https://doi.org/10.1038/S41586-021-03985-3
