Vascularized organoids on a chip: strategies for engineering organoids with functional vasculature
Name
d0lc01186j.pdf
Description
Published version
Size
5.22 MB
Format
Adobe PDF
Checksum (MD5)
9cb0b8b4b9d162f75e944083d95a7a7b
Author(s)
Zhang, Shun
Wan, Zhengpeng
Kamm, Roger D
Date Issued
2021
Journal
Lab on a Chip
Publisher
Royal Society of Chemistry (RSC)
Version
Final published version
Abstract
© The Royal Society of Chemistry 2021. Human organoids, self-organized and differentiated from homogenous pluripotent stem cells (PSC), replicate the key structural and functional characteristics of theirin vivocounterparts. Despite the rapid advancement of organoid technology and its diverse applications, major limitations in achieving trulyin vivolike functionality have been the lack of matured structural organization and constraints on tissue size, both of which are direct consequences of lacking a functional vasculature. In the absence of perfusable vessels, a core region within organoids quickly becomes necrotic during development due to increased metabolic demands that cannot be met by diffusion alone. Thus, incorporating functional vasculature in organoid models is indispensable for their growth in excess of several hundred microns and maturaturation beyond the embryonic and fetal phase. Here, we review recent advancements in vascularizing organoids and engineeringin vitrocapillary beds, and further explore strategies to integrate them on a microfluidic based platform, aiming for establishing perfused vasculature throughout organoidsin vitro.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Mechanical Engineering
Terms of Use
Creative Commons Attribution 3.0 unported license
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1039/d0lc01186j
