A Reporter Screen in a Human Haploid Cell Line Identifies CYLD as a Constitutive Inhibitor of NF-κB
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Lee-2013-A Reporter Screen in.pdf
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Author(s)
Carette, Jan E.
Brummelkamp, Thijn R.
Lee, Clarissa Ci
Ploegh, Hidde
Date Issued
July 2013
Journal
PLoS ONE
Publisher
Public Library of Science
Citation
Lee, Clarissa C., Jan E. Carette, Thijn R. Brummelkamp, and Hidde L. Ploegh. “A Reporter Screen in a Human Haploid Cell Line Identifies CYLD as a Constitutive Inhibitor of NF-κB.” Edited by Alexander Poltorak. PLoS ONE 8, no. 7 (July 8, 2013): e70339.
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Final published version
Abstract
The development of forward genetic screens in human haploid cells has the potential to transform our understanding of the genetic basis of cellular processes unique to man. So far, this approach has been limited mostly to the identification of genes that mediate cell death in response to a lethal agent, likely due to the ease with which this phenotype can be observed. Here, we perform the first reporter screen in the near-haploid KBM7 cell line to identify constitutive inhibitors of NF-κB. CYLD was the only currently known negative regulator of NF-κB to be identified, thus uniquely distinguishing this gene. Also identified were three genes with no previous known connection to NF-κB. Our results demonstrate that reporter screens in haploid human cells can be applied to investigate the many complex signaling pathways that converge upon transcription factors.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
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DOI of Published Version
https://doi.org/10.1371/journal.pone.0070339
