A structural phylogeny for understanding 2-oxoacid oxidoreductase function
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nihms791146.pdf
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Author(s)
Gibson, Marcus Ian
Chen, Yang-Ting
Drennan, Catherine L.
Date Issued
June 2016
Journal
Current Opinion in Structural Biology
Publisher
Elsevier BV
Citation
Gibson, Marcus I et al. “A Structural Phylogeny for Understanding 2-Oxoacid Oxidoreductase Function.” Current Opinion in Structural Biology 41 (December 2016): 54–61 © 2016 Elsevier Ltd
Version
Author's final manuscript
Abstract
2-Oxoacid:ferredoxin oxidoreductases (OFORs) are essential enzymes in microbial one-carbon metabolism. They use thiamine pyrophosphate to reversibly cleave carbon-carbon bonds, generating low potential (~-500 mV) electrons. Crystallographic analysis of a recently discovered OFOR, an oxalate oxidoreductase (OOR), has provided a second view of OFOR architecture and active site composition. Using these recent structural data along with the previously determined structures of pyruvate:ferredoxin oxidoreductase, structure-function relationships in this superfamily have been expanded and re-evaluated. Additionally, structural motifs have been defined that better serve to distinguish one OFOR subfamily from another and potentially uncover novel OFORs.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Department of Chemistry
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
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DOI of Published Version
https://doi.org/10.1016/J.SBI.2016.05.011
