Chiral Antioxidant-based Gold Nanoclusters Reprogram DNA Epigenetic Patterns
Name
Chiral antioxidant.pdf
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1.72 MB
Format
Adobe PDF
Checksum (MD5)
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Author(s)
Ma, Yue
Fu, Hualin
Zhang, Chunlei
Cheng, Shangli
Gao, Jie
Wang, Zhen
Jin, Weilin
Cui, Daxiang
Osorio De Castro Conde, Joao
Date Issued
April 2016
Journal
Scientific Reports
Publisher
Nature Publishing Group
Citation
Ma, Yue et al. “Chiral Antioxidant-Based Gold Nanoclusters Reprogram DNA Epigenetic Patterns.” Scientific Reports 6.1 (2016): n. pag.
Version
Final published version
Abstract
Epigenetic modifications sit ‘on top of’ the genome and influence DNA transcription, which can force a significant impact on cellular behavior and phenotype and, consequently human development and disease. Conventional methods for evaluating epigenetic modifications have inherent limitations and, hence, new methods based on nanoscale devices are needed. Here, we found that antioxidant (glutathione) chiral gold nanoclusters induce a decrease of 5-hydroxymethylcytosine (5hmC), which is an important epigenetic marker that associates with gene transcription regulation. This epigenetic change was triggered partially through ROS activation and oxidation generated by the treatment with glutathione chiral gold nanoclusters, which may inhibit the activity of TET proteins catalyzing the conversion of 5-methylcytosine (5mC) to 5hmC. In addition, these chiral gold nanoclusters can downregulate TET1 and TET2 mRNA expression. Alteration of TET-5hmC signaling will then affect several downstream targets and be involved in many aspects of cell behavior. We demonstrate for the first time that antioxidant-based chiral gold nanomaterials have a direct effect on epigenetic process of TET-5hmC pathways and reveal critical DNA demethylation patterns.
MIT Department
Institute for Medical Engineering and Science
Harvard University--MIT Division of Health Sciences and Technology
Terms of Use
Creative Commons Attribution 4.0 International License
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DOI of Published Version
https://doi.org/10.1038/srep33436
