Transcription factor trapping by RNA in gene regulatory elements
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Sharp_Transcription factor.pdf
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Author(s)
Sigova, A. A.
Abraham, Brian Joseph
Ji, X.
Molinie, B.
Hannett, N. M.
Guo, Y. E.
Giallourakis, C. C.
Jangi, Mohini Rohit
Sharp, Phillip A.
Young, Richard A.
Date Issued
November 2015
Journal
Science
Publisher
American Association for the Advancement of Science (AAAS)
Citation
Sigova, A. A. et al. “Transcription Factor Trapping by RNA in Gene Regulatory Elements.” Science 350.6263 (2015): 978–981.
Version
Author's final manuscript
Abstract
Transcription factors (TFs) bind specific sequences in promoter-proximal and -distal DNA elements to regulate gene transcription. RNA is transcribed from both of these DNA elements, and some DNA binding TFs bind RNA. Hence, RNA transcribed from regulatory elements may contribute to stable TF occupancy at these sites. We show that the ubiquitously expressed TF Yin-Yang 1 (YY1) binds to both gene regulatory elements and their associated RNA species across the entire genome. Reduced transcription of regulatory elements diminishes YY1 occupancy, whereas artificial tethering of RNA enhances YY1 occupancy at these elements. We propose that RNA makes a modest but important contribution to the maintenance of certain TFs at gene regulatory elements and suggest that transcription of regulatory elements produces a positive-feedback loop that contributes to the stability of gene expression programs.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
Koch Institute for Integrative Cancer Research at MIT
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1126/science.aad3346
