Controlled electromechanical cell stimulation on-a-chip
Name
Pavesi-2015-Controlled electromechanical.pdf
Size
1.34 MB
Format
Adobe PDF
Checksum (MD5)
55fb007ca103c069155c8c2952db5b83
Author(s)
Pavesi, Andrea
Adriani, Giulia
Rasponi, Marco
Zervantonakis, Ioannis K.
Fiore, Gianfranco B.
Kamm, Roger Dale
Date Issued
July 2015
Journal
Scientific Reports
Publisher
Nature Publishing Group
Citation
Pavesi, Andrea, Giulia Adriani, Marco Rasponi, Ioannis K. Zervantonakis, Gianfranco B. Fiore, and Roger D. Kamm. “Controlled Electromechanical Cell Stimulation on-a-Chip.” Scientific Reports 5 (July 2, 2015): 11800.
Version
Final published version
Abstract
Stem cell research has yielded promising advances in regenerative medicine, but standard assays generally lack the ability to combine different cell stimulations with rapid sample processing and precise fluid control. In this work, we describe the design and fabrication of a micro-scale cell stimulator capable of simultaneously providing mechanical, electrical, and biochemical stimulation, and subsequently extracting detailed morphological and gene-expression analysis on the cellular response. This micro-device offers the opportunity to overcome previous limitations and recreate critical elements of the in vivo microenvironment in order to investigate cellular responses to three different stimulations. The platform was validated in experiments using human bone marrow mesenchymal stem cells. These experiments demonstrated the ability for inducing changes in cell morphology, cytoskeletal fiber orientation and changes in gene expression under physiological stimuli. This novel bioengineering approach can be readily applied to various studies, especially in the fields of stem cell biology and regenerative medicine.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Mechanical Engineering
Terms of Use
Creative Commons Attribution
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/srep11800
