Lipid-like Self-Assembling Peptide Nanovesicles for Drug Delivery
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Fatouros-2014-Lipid-like self.pdf
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Author(s)
Fatouros, Dimitrios G.
Lamprou, Dimitrios A.
Urquhart, Andrew J.
Yannopoulos, Spyros N.
Vizirianakis, Ioannis S.
Zhang, Shuguang
Koutsopoulos, Sotirios
Date Issued
May 2014
Journal
ACS Applied Materials & Interfaces
Publisher
American Chemical Society (ACS)
Citation
Fatouros, Dimitrios G., Dimitrios A. Lamprou, Andrew J. Urquhart, Spyros N. Yannopoulos, Ioannis S. Vizirianakis, Shuguang Zhang, and Sotirios Koutsopoulos. “Lipid-Like Self-Assembling Peptide Nanovesicles for Drug Delivery.” ACS Applied Materials & Interfaces 6, no. 11 (June 11, 2014): 8184–8189. © 2014 American Chemical Society
Version
Final published version
Abstract
Amphiphilic self-assembling peptides are functional materials, which, depending on the amino acid sequence, the peptide length, and the physicochemical conditions, form a variety of nanostructures including nanovesicles, nanotubes, and nanovalves. We designed lipid-like peptides with an aspartic acid or lysine hydrophilic head and a hydrophobic tail composed of six alanines (i.e., ac-A[subscript 6]K-CONH[subscript 2], KA[subscript 6]-CONH[subscript 2], ac-A[subscript 6]D-COOH, and DA[subscript 6]-COOH). The resulting novel peptides have a length similar to biological lipids and form nanovesicles at physiological conditions. AFM microscopy and light scattering analyses of the positively charged lipid-like ac-A[subscript 6]K-CONH[subscript 2], KA[subscript 6]-CONH[subscript 2] peptide formulations showed individual nanovesicles. The negatively charged ac-A[subscript 6]D-COOH and DA[subscript 6]-COOH peptides self-assembled into nanovesicles that formed clusters that upon drying were organized into necklace-like formations of nanovesicles. Encapsulation of probe molecules and release studies through the peptide bilayer suggest that peptide nanovesicles may be good candidates for sustained release of pharmaceutically active hydrophilic and hydrophobic compounds. Lipid-like peptide nanovesicles represent a paradigm shifting system that may complement liposomes for the delivery of diagnostic and therapeutic agents.
MIT Department
Massachusetts Institute of Technology. Center for Biomedical Engineering
Massachusetts Institute of Technology. Center for Bits and Atoms
Massachusetts Institute of Technology. Media Laboratory
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DOI of Published Version
https://doi.org/10.1021/am501673x
