Convergence of case-specific epigenetic alterations identify a confluence of genetic vulnerabilities tied to opioid overdose
Name
nihms-1778910.pdf
Description
Accepted version
Size
1.64 MB
Format
Adobe PDF
Checksum (MD5)
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Author(s)
Corradin, Olivia
Sallari, Richard
Hoang, An T
Kassim, Bibi S
Ben Hutta, Gabriella
Cuoto, Lizette
Quach, Bryan C
Lovrenert, Katreya
Hays, Cameron
Gryder, Berkley E
Date Issued
2022
Journal
Molecular Psychiatry
Publisher
Springer Science and Business Media LLC
Citation
Corradin, Olivia, Sallari, Richard, Hoang, An T, Kassim, Bibi S, Ben Hutta, Gabriella et al. 2022. "Convergence of case-specific epigenetic alterations identify a confluence of genetic vulnerabilities tied to opioid overdose." Molecular Psychiatry, 27 (4).
Version
Author's final manuscript
Abstract
Opioid use disorder is a highly heterogeneous disease driven by a variety of genetic and environmental risk factors which have yet to be fully elucidated. Opioid overdose, the most severe outcome of opioid use disorder, remains the leading cause of accidental death in the United States. We interrogated the effects of opioid overdose on the brain using ChIP-seq to quantify patterns of H3K27 acetylation in dorsolateral prefrontal cortical neurons isolated from 51 opioid-overdose cases and 51 accidental death controls. Among opioid cases, we observed global hypoacetylation and identified 388 putative enhancers consistently depleted for H3K27ac. Machine learning on H3K27ac patterns predicted case-control status with high accuracy. We focused on case-specific regulatory alterations, revealing 81,399 hypoacetylation events, uncovering vast inter-patient heterogeneity. We developed a strategy to decode this heterogeneity based on convergence analysis, which leveraged promoter-capture Hi-C to identify five genes over-burdened by alterations in their regulatory network or "plexus": ASTN2, KCNMA1, DUSP4, GABBR2, ENOX1. These convergent loci are enriched for opioid use disorder risk genes and heritability for generalized anxiety, number of sexual partners, and years of education. Overall, our multi-pronged approach uncovers neurobiological aspects of opioid use disorder and captures genetic and environmental factors perpetuating the opioid epidemic.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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DOI of Published Version
https://doi.org/10.1038/S41380-022-01477-Y
