Control of Development by Altered Localization of a Transcription Factor in B. subtilis
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Grossman_Control of development.pdf
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Author(s)
Lin, Daniel Chi-Hong
Quisel, John D.
Grossman, Alan Davis
Date Issued
November 1999
Journal
Molecular Cell
Publisher
Elsevier
Citation
Quisel, John D, Daniel Chi-Hong Lin, and Alan D Grossman. “Control of Development by Altered Localization of a Transcription Factor in B. subtilis.” Molecular Cell 4, no. 5 (November 1999): 665-672. Copyright © 1999 Cell Press.
Version
Final published version
Abstract
In B. subtilis, the chromosome partitioning proteins Soj (ParA) and Spo0J (ParB) regulate the initiation of sporulation. Soj is a negative regulator of sporulation gene expression, and Spo0J antagonizes Soj function. Using fusions of Soj to green fluorescent protein, we found that Soj localized near the cell poles and upon entry into stationary phase oscillated from pole to pole. In the absence of Spo0J, Soj was associated predominantly with DNA. By in vivo cross-linking and immunoprecipitation, we found that Soj physically associates with developmentally regulated promoters, and this association increased in the absence of Spo0J. These results show that Soj switches localization and function depending on the chromosome partitioning protein Spo0J. We further show that mutations in the Soj ATPase domain disrupt localization and function and render Soj insensitive to regulation by Spo0J.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1016/S1097-2765(00)80377-9
