Lipidoid-Coated Iron Oxide Nanoparticles for Efficient DNA and siRNA delivery
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Author(s)
Jiang, Shan
Eltoukhy, Ahmed A.
Anderson, Daniel Griffith
Love, Kevin T
Langer, Robert S
Date Issued
March 2013
Journal
Nano Letters
Publisher
American Chemical Society (ACS)
Citation
Jiang, Shan, Ahmed A. Eltoukhy, Kevin T. Love, Robert Langer, and Daniel G. Anderson. “Lipidoid-Coated Iron Oxide Nanoparticles for Efficient DNA and siRNA Delivery.” Nano Lett. 13, no. 3 (March 13, 2013): 1059–1064.
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Author's final manuscript
Abstract
The safe, targeted and effective delivery of gene therapeutics remains a significant barrier to their broad clinical application. Here we develop a magnetic nucleic acid delivery system composed of iron oxide nanoparticles and cationic lipid-like materials termed lipidoids. Coated nanoparticles are capable of delivering DNA and siRNA to cells in culture. The mean hydrodynamic size of these nanoparticles was systematically varied and optimized for delivery. While nanoparticles of different sizes showed similar siRNA delivery efficiency, nanoparticles of 50–100 nm displayed optimal DNA delivery activity. The application of an external magnetic field significantly enhanced the efficiency of nucleic acid delivery, with performance exceeding that of the commercially available lipid-based reagent, Lipofectamine 2000. The iron oxide nanoparticle delivery platform developed here offers the potential for magnetically guided targeting, as well as an opportunity to combine gene therapy with MRI imaging and magnetic hyperthermia.
MIT Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Massachusetts Institute of Technology. Department of Chemical Engineering
Koch Institute for Integrative Cancer Research at MIT
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DOI of Published Version
https://doi.org/10.1021/nl304287a
