Alteration of processing induced by a single nucleotide polymorphism in pri-miR-126
Name
Lodish_Alteration of processing.pdf
Size
571.99 KB
Format
Adobe PDF
Checksum (MD5)
a2885d1b84d0c420a219645e54157015
Author(s)
Harnprasopwat, Ratanakanit
Ha, Daon
Toyoshima, Takae
Tojo, Arinobu
Kotani, Ai
Lodish, Harvey F
Date Issued
July 2010
Journal
Biochemical and Biophysical Research Communications
Publisher
Elsevier
Citation
Harnprasopwat, Ratanakanit, Daon Ha, Takae Toyoshima, Harvey Lodish, Arinobu Tojo, and Ai Kotani. “Alteration of Processing Induced by a Single Nucleotide Polymorphism in Pri-miR-126.” Biochemical and Biophysical Research Communications 399, no. 2 (August 2010): 117–122.
Version
Author's final manuscript
Abstract
MicroRNAs (miRNAs) are small non-coding RNAs that inhibit expression of specific target genes at the post-transcriptional level. Sequence variations in miRNA genes, including pri-miRNAs, pre-miRNAs and mature miRNAs, could potentially influence the processing and/or target selection of miRNAs. In this study, we have found the single nucleotide polymorphism (SNP) at the twenty-fourth nucleotide (+24) of the mature miR-126 in the genome of RS4;11 cells, derived from a MLL-AF4 ALL patient. Through a series of in vivo analyzes, we found that this miR-126 SNP significantly blocks the processing of pri-miRNA to mature miRNA, as well as reduces miRNA-mediated translational suppression. Moreover, its frequency is different among races. Thus, our study emphasizes the importance of identifying new miRNA SNP and its contribution to miRNA biogenesis which is possible link to human genetic disease.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
Terms of Use
Creative Commons Attribution-Noncommercial-NoDerivatives
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/j.bbrc.2010.07.009
