Cyanophycin Mediates the Accumulation and Storage of Fixed Carbon in Non-Heterocystous Filamentous Cyanobacteria from Coniform Mats
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Liang-2014-Cyanophycin Mediates.pdf
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Author(s)
Liang, Biqing
Wu, Ting-Di
Sun, Hao-Jhe
Vali, Hojatollah
Guerquin-Kern, Jean-Luc
Wang, Chung-Ho
Bosak, Tanja
Date Issued
February 2014
Journal
PLoS ONE
Publisher
Public Library of Science
Citation
Liang, Biqing, Ting-Di Wu, Hao-Jhe Sun, Hojatollah Vali, Jean-Luc Guerquin-Kern, Chung-Ho Wang, and Tanja Bosak. “Cyanophycin Mediates the Accumulation and Storage of Fixed Carbon in Non-Heterocystous Filamentous Cyanobacteria from Coniform Mats.” Edited by Brett Neilan. PLoS ONE 9, no. 2 (February 7, 2014): e88142.
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Final published version
Abstract
Thin, filamentous, non-heterocystous, benthic cyanobacteria (Subsection III) from some marine, lacustrine and thermal environments aggregate into macroscopic cones and conical stromatolites. We investigate the uptake and storage of inorganic carbon by cone-forming cyanobacteria from Yellowstone National Park using high-resolution stable isotope mapping of labeled carbon (H13CO3−) and immunoassays. Observations and incubation experiments in actively photosynthesizing enrichment cultures and field samples reveal the presence of abundant cyanophycin granules in the active growth layer of cones. These ultrastructurally heterogeneous granules rapidly accumulate newly fixed carbon and store 18% of the total particulate labeled carbon after 120 mins of incubation. The intracellular distribution of labeled carbon during the incubation experiment demonstrates an unexpectedly large contribution of PEP carboxylase to carbon fixation, and a large flow of carbon and nitrogen toward cyanophycin in thin filamentous, non-heterocystous cyanobacteria. This pattern does not occur in obvious response to a changing N or C status. Instead, it may suggest an unusual interplay between the regulation of carbon concentration mechanisms and accumulation of photorespiratory products that facilitates uptake of inorganic C and reduces photorespiration in the dense, surface-attached communities of cyanobacteria from Subsection III.
MIT Department
Massachusetts Institute of Technology. Department of Earth, Atmospheric, and Planetary Sciences
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DOI of Published Version
https://doi.org/10.1371/journal.pone.0088142
