Continuous and Long-Term Volume Measurements with a Commercial Coulter Counter
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Bryan-2012-Continuous and Long-.pdf
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871.23 KB
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Author(s)
Bryan, Andrea Kristine
Engler, Alex
Gulati, Amneet
Manalis, Scott R
Date Issued
January 2012
Journal
PLoS ONE
Publisher
Public Library of Science
Citation
Bryan, Andrea K. et al. “Continuous and Long-Term Volume Measurements with a Commercial Coulter Counter.” Ed. Michael Polymenis. PLoS ONE 7.1 (2012): e29866. Web. 27 June 2012.
Version
Final published version
Abstract
We demonstrate a method to enhance the time resolution of a commercial Coulter counter and enable continuous and long-term cell size measurements for growth rate analyses essential to understanding basic cellular processes, such as cell size regulation and cell cycle progression. Our simple modifications to a commercial Coulter counter create controllable cell culture conditions within the sample compartment and combine temperature control with necessary adaptations to achieve measurement stability over several hours. We also wrote custom software, detailed here, to analyze instrument data files collected by either this continuous method or standard, periodic sampling. We use the continuous method to measure the growth rate of yeast in G1 during a prolonged arrest and, in different samples, the dependency of growth rate on cell size and cell cycle position in arrested and proliferating cells. We also quantify with high time resolution the response of mouse lymphoblast cell culture to drug treatment. This method provides a technique for continuous measurement of cell size that is applicable to a large variety of cell types and greatly expands the set of analysis tools available for the Coulter counter.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Koch Institute for Integrative Cancer Research at MIT
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DOI of Published Version
https://doi.org/10.1371/journal.pone.0029866
