Iterative expansion microscopy
Name
nihms862423.pdf
Description
Accepted version
Size
2.02 MB
Format
Adobe PDF
Checksum (MD5)
eee5b8901ea488d07ac3c60a3895ec26
Author(s)
Chang, Jae-Byum
Chen, Fei
Yoon, Young-Gyu
Jung, Erica E
Babcock, Hazen
Kang, Jeong Seuk
Asano, Shoh
Suk, Ho-Jun
Pak, Nikita
Tillberg, Paul W
Date Issued
2017
Journal
Nature Methods
Publisher
Springer Nature
Version
Author's final manuscript
Abstract
© 2017 Nature America, Inc. All rights reserved. We recently developed a method called expansion microscopy, in which preserved biological specimens are physically magnified by embedding them in a densely crosslinked polyelectrolyte gel, anchoring key labels or biomolecules to the gel, mechanically homogenizing the specimen, and then swelling the gel-specimen composite by ∼4.5× in linear dimension. Here we describe iterative expansion microscopy (iExM), in which a sample is expanded ∼20×. After preliminary expansion a second swellable polymer mesh is formed in the space newly opened up by the first expansion, and the sample is expanded again. iExM expands biological specimens ∼4.5 × 4.5, or ∼20×, and enables ∼25-nm-resolution imaging of cells and tissues on conventional microscopes. We used iExM to visualize synaptic proteins, as well as the detailed architecture of dendritic spines, in mouse brain circuitry.
MIT Department
Massachusetts Institute of Technology. Media Laboratory
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Mechanical Engineering
McGovern Institute for Brain Research at MIT
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1038/NMETH.4261
