Investigating Effects of Proteasome Inhibitor on Multiple Myeloma Cells Using Confocal Raman Microscopy
Name
Investigating effects.pdf
Size
1.5 MB
Format
Adobe PDF
Checksum (MD5)
13ceff4937d1c1a18bcae141c190a475
Author(s)
Kang, Jeon Woong
Singh, Surya Pratap
Nguyen, Freddy T.
Lue, Niyom
Sung, Yongjin
So, Peter T. C.
Dasari, Ramachandra Rao
Date Issued
December 2016
Journal
Sensors
Publisher
MDPI AG
Citation
Kang, Jeon et al. “Investigating Effects of Proteasome Inhibitor on Multiple Myeloma Cells Using Confocal Raman Microscopy.” Sensors 16.12 (2016): 2133.
Version
Final published version
Abstract
Due to its label-free and non-destructive nature, applications of Raman spectroscopic imaging in monitoring therapeutic responses at the cellular level are growing. We have recently developed a high-speed confocal Raman microscopy system to image living biological specimens with high spatial resolution and sensitivity. In the present study, we have applied this system to monitor the effects of Bortezomib, a proteasome inhibitor drug, on multiple myeloma cells. Cluster imaging followed by spectral profiling suggest major differences in the nuclear and cytoplasmic contents of cells due to drug treatment that can be monitored with Raman spectroscopy. Spectra were also acquired from group of cells and feasibility of discrimination among treated and untreated cells using principal component analysis (PCA) was accessed. Findings support the feasibility of Raman technologies as an alternate, novel method for monitoring live cell dynamics with minimal external perturbation.
MIT Department
Lincoln Laboratory
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Department of Chemistry
Massachusetts Institute of Technology. Department of Mechanical Engineering
Massachusetts Institute of Technology. Laser Biomedical Research Center
Terms of Use
Creative Commons Attribution 4.0 International License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.3390/s16122133
