Light-Triggered Myosin Activation for Probing Dynamic Cellular Processes
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Imperiali5_Goguen_ACIE_2011.pdf
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1.21 MB
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Checksum (MD5)
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Author(s)
Goguen, Brenda N.
Hoffman, Brenton D.
Sellers, James R.
Schwartz, Martin A.
Imperiali, Barbara
Date Issued
June 2011
Journal
Angewandte Chemie International Edition
Publisher
Wiley-VCH Verlag
Citation
Goguen, Brenda N. et al. “Light-Triggered Myosin Activation for Probing Dynamic Cellular Processes.” Angewandte Chemie International Edition 50.25 (2011): 5667–5670.
Version
Author's final manuscript
Abstract
Shining light on myosin: The incorporation of a caging group onto the essential phosphoserine residue of myosin by protein semisynthesis enables light-triggered activation of the protein (see picture). Caging eliminates the myosin activity, but exposure to 365 nm light restores its function to native levels. The caged protein can also be introduced into cells to facilitate studies of myosin with precise spatial and temporal resolution.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Department of Chemistry
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Creative Commons Attribution-Noncommercial-Share Alike 3.0
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DOI of Published Version
https://doi.org/10.1002/anie.201100674
