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Methanogen Productivity and Microbial Community Composition Varies With Iron Oxide Mineralogy
Name
fmicb-12-705501.pdf
Description
Published version
Size
3.76 MB
Format
Adobe PDF
Checksum (MD5)
6979cab306a9d1527d4564dc2f4414df
Author(s)
Gadol, Hayley J
Elsherbini, Joseph
Kocar, Benjamin D
Date Issued
2021
Journal
Frontiers in Microbiology
Publisher
Frontiers Media SA
Citation
Gadol, Hayley J, Elsherbini, Joseph and Kocar, Benjamin D. 2021. "Methanogen Productivity and Microbial Community Composition Varies With Iron Oxide Mineralogy." Frontiers in Microbiology, 12.
Version
Final published version
Abstract
Quantifying the flux of methane from terrestrial environments remains challenging, owing to considerable spatial and temporal variability in emissions. Amongst a myriad of factors, variation in the composition of electron acceptors, including metal (oxyhydr)oxides, may impart controls on methane emission. The purpose of this research is to understand how iron (oxyhydr)oxide minerals with varied physicochemical properties influence microbial methane production and subsequent microbial community development. Incubation experiments, using lake sediment as an inoculum and acetate as a carbon source, were used to understand the influence of one poorly crystalline iron oxide mineral, ferrihydrite, and two well-crystalline minerals, hematite and goethite, on methane production. Iron speciation, headspace methane, and 16S-rRNA sequencing microbial community data were measured over time. Substantial iron reduction only occurred in the presence of ferrihydrite while hematite and goethite had little effect on methane production throughout the incubations. In ferrihydrite experiments the time taken to reach the maximum methane production rate was slower than under other conditions, but methane production, eventually occurred in the presence of ferrihydrite. We suggest that this is due to ferrihydrite transformation into more stable minerals like magnetite and goethite or surface passivation by Fe(II). While all experimental conditions enriched for Methanosarcina, only the presence of ferrihydrite enriched for iron reducing bacteria Geobacter. Additionally, the presence of ferrihydrite continued to influence microbial community development after the onset of methanogenesis, with the dissimilarity between communities growing in ferrihydrite compared to no-Fe-added controls increasing over time. This work improves our understanding of how the presence of different iron oxides influences microbial community composition and methane production in soils and sediments.
Terms of Use
Creative Commons Attribution 4.0 International license
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DOI of Published Version
10.3389/fmicb.2021.705501
