Direct observation of mammalian cell growth and size regulation
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Manalis_Direct observation.pdf
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Author(s)
Son, Sungmin
Tzur, Amit
Weng, Yaochung
Jorgensen, Paul
Kim, Jisoo
Kirschner, Marc W.
Manalis, Scott R
Date Issued
August 2012
Journal
Nature Methods
Publisher
Nature Publishing Group
Citation
Son, Sungmin, Amit Tzur, Yaochung Weng, Paul Jorgensen, Jisoo Kim, Marc W Kirschner, and Scott R Manalis. “Direct Observation of Mammalian Cell Growth and Size Regulation.” Nature Methods 9, no. 9 (August 5, 2012): 910–912.
Version
Author's final manuscript
Abstract
We introduce a microfluidic system for simultaneously measuring single-cell mass and cell cycle progression over multiple generations. We use this system to obtain over 1,000 h of growth data from mouse lymphoblast and pro–B-cell lymphoid cell lines. Cell lineage analysis revealed a decrease in the growth rate variability at the G1-S phase transition, which suggests the presence of a growth rate threshold for maintaining size homeostasis.
MIT Department
Massachusetts Institute of Technology. Computational and Systems Biology Program
Massachusetts Institute of Technology. Department of Mechanical Engineering
Koch Institute for Integrative Cancer Research at MIT
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
http://dx.doi.org/10.1038/nmeth.2133
