Selective single cell detachment and retrieval for downstream analyses using nanosecond laser pulses in cnt-coated microwell arrays
Name
Laser_Extended_Abs_v2.pdf
Size
3.18 MB
Format
Adobe PDF
Checksum (MD5)
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Author(s)
Chen, Yu-Chih
Baac, Hyoung Won
Lee, Kyu-Tae
Teichert, Kendall
Guo, L. Jay
Yoon, Euisik
Hart, Anastasios John
Date Issued
October 2015
Journal
19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher
Transducers Research Foundation, Inc.
Citation
Chen, Yu-Chih, Hyoung Won Baac, Kyu-Tae Lee, Kendall Teichert, A. John Hart, L. Jay Guo and Euisik Yoon. "Selective single cell detachment and retrieval for downstream analyses using nanosecond laser pulses in cnt-coated microwell arrays." 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, October 25-29, 2015, Gyeongju, Korea.
Version
Author's final manuscript
Abstract
Cellular heterogeneity is one of the key hallmarks in cancer biology, but conventional dish-based assays only report the average behavior of many cells. Microfluidics can facilitate manipulating and monitoring of individual cells, yet it is difficult to retrieve a specific target single cell from an enclosed microfluidic chip. In this work, we have successfully developed a selective cell detachment and retrieval scheme with a spatial resolution of around 10μm. The retrieved cells were proved to be viable, and the detachment process has negligible effect on membrane proteins and mRNA expression, providing an ideal tools for the downstream analysis of target cells.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
http://www.rsc.org/images/LOC/2015/PDFs/Papers/0224_3B2-2.pdf
