The interplay between asymmetric and symmetric DNA loop extrusion
Name
elife-63528-v2.pdf
Description
Published version
Size
1.77 MB
Format
Adobe PDF
Checksum (MD5)
ab4d8785d851ab3823eb95dd05d890f0
Author(s)
Banigan, Edward J
Mirny, Leonid A
Date Issued
2020
Journal
eLife
Publisher
eLife Sciences Publications, Ltd
Citation
Banigan, Edward J and Mirny, Leonid A. 2020. "The interplay between asymmetric and symmetric DNA loop extrusion." eLife, 9.
Version
Final published version
Abstract
© Banigan and Mirny. Chromosome compaction is essential for reliable transmission of genetic information. Experiments suggest that -1000-fold compaction is driven by condensin complexes that extrude chromatin loops, by progressively collecting chromatin fiber from one or both sides of the complex to form a growing loop. Theory indicates that symmetric two-sided loop extrusion can achieve such compaction, but recent single-molecule studies (Golfier et al., 2020) observed diverse dynamics of condensins that perform one-sided, symmetric two-sided, and asymmetric two-sided extrusion. We use simulations and theory to determine how these molecular properties lead to chromosome compaction. High compaction can be achieved if even a small fraction of condensins have two essential properties: A long residence time and the ability to perform two-sided (not necessarily symmetric) extrusion. In mixtures of condensins I and II, coupling two-sided extrusion and stable chromatin binding by condensin II promotes compaction. These results provide missing connections between single-molecule observations and chromosome-scale organization.
MIT Department
Massachusetts Institute of Technology. Department of Physics
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Terms of Use
Creative Commons Attribution 4.0 International license
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.7554/ELIFE.63528
