Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling
Author(s)
Uttamapinant, Chayasith
Tangpeerachaikul, Anupong
Grecian, Scott
Clarke, Scott M.
Singh, Upinder
Slade, Peter
Gee, Kyle R.
Ting, Alice Y.
Date Issued
May 2012
Journal
Angewandte Chemie International Edition
Publisher
Wiley Blackwell
Citation
Uttamapinant, Chayasith et al. “Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling.” Angewandte Chemie International Edition 51.24 (2012): 5852–5856.
Version
Author's final manuscript
Abstract
Bring your own copper: Copper-chelating azides undergo much faster click reactions (CuAAC) than nonchelating azides under a variety of biocompatible conditions. This kinetic enhancement allows site-specific protein labeling to be performed on the surface of living cells with only 10–40 μM Cu[superscript I]/Cu[superscript II] (see scheme). Detection sensitivity was also increased for CuAAC detection of alkyne-modified proteins and RNA.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike 3.0
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1002/anie.201108181
