Non-viral delivery of CRISPR/Cas9 complex using CRISPR-GPS nanocomplexes
Name
c9nr01786k.pdf
Description
Published version
Size
2.07 MB
Format
Adobe PDF
Checksum (MD5)
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Author(s)
Jain, Piyush Kumar
Lo, Justin H.
Rananaware, Santosh
Downing, Marco
Panda, Apekshya
Tai, Michelle
Raghavan, Srivatsan
Fleming, Heather
Bhatia, Sangeeta N
Date Issued
November 2019
Journal
Nanoscale
Publisher
Royal Society of Chemistry (RSC)
Citation
Jain, Piyush K. et al. "Non-viral delivery of CRISPR/Cas9 complex using CRISPR-GPS nanocomplexes." Nanoscale, 11, 44 (November 2019): 21317-21323 © 2019 The Royal Society of Chemistry
Version
Final published version
Abstract
There is a critical need for the development of safe and efficient delivery technologies for CRISPR/Cas9
to advance translation of genome editing to the clinic. Non-viral methods that are simple, efficient, and
completely based on biologically-derived materials could offer such potential. Here we report a simple
and modular tandem peptide-based nanocomplex system with cell-targeting capacity that efficiently
combines guide RNA (sgRNA) with Cas9 protein, and facilitates internalization of sgRNA/Cas9 ribonucleoprotein complexes to yield robust genome editing across multiple cell lines
Subjects
General Materials Science
MIT Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Chemical Engineering
Broad Institute of MIT and Harvard
Terms of Use
Creative Commons Attribution Noncommercial 3.0 unported license
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1039/c9nr01786k
