Online Raman spectroscopy for bioprocess monitoring
Name
78616168-MIT.pdf
Description
Full printable version
Size
12.06 MB
Format
Adobe PDF
Checksum (MD5)
139fc231196270d9cf6ad4d0b2a1f3ff
Author(s)
Gil, Gustavo Adolfo
Advisor(s)
Rajeev Ram.
Date Issued
2005
Publisher
Massachusetts Institute of Technology
Abstract
Online monitoring of bioprocesses is essential to expanding the potential of biotechnology. In this thesis, a system to estimate concentrations of chemical components of an Escherichia Coli fermentation growth medium via a remote fiber-optic Raman spectroscopy probe was studied in depth. The system was characterized to determine sources of instability and systematic error. A complete first-order error analysis was conducted to determine the theoretical sensitivity of the instrument. A suite of improvements and new features, including an online estimation of optical density and biomass, a method to correct for wavelength shifts, and a setup to increase repeatability and throughput for offline and calibration methods was developed accordingly. The theoretical and experimental ground work for developing a correction for spectrum distortions caused by elastic scattering, a fundamental problem for many spectroscopic applications, was laid out. In addition, offline Raman spectroscopy was used to estimate concentrations of fructose, glucose, sucrose, and nitrate in an oil palm (Elais guineensis) bioreaction. Finally, an expansion of optical techniques into new scale-up applications in plant cell bioprocesses, such as plant call differentiation was explored.
Description
Thesis (M. Eng. and S.B.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2005.
Author received the S.B. degree, June 2005 and the M. Eng. degree, Sept. 2005.
Includes bibliographical references (p. 195-202).
Subjects
Electrical Engineering and Computer Science.
MIT Department
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Terms of Use
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