Applications of the CRISPR–Cas9 system in cancer biology
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nihms712031.pdf
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2.53 MB
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Adobe PDF
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Author(s)
Sanchez-Rivera, Francisco Javier
Jacks, Tyler E.
Date Issued
June 2015
Journal
Nature Reviews Cancer
Publisher
Nature Publishing Group
Citation
Sánchez-Rivera et al. “Applications of the CRISPR–Cas9 System in Cancer Biology.” Nature Reviews Cancer 15, 7 (June 2015): 387–393 © 2015 Macmillan Publishers Limited
Version
Author's final manuscript
Abstract
The prokaryotic type II CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-CRISPR-associated 9) system is rapidly revolutionizing the field of genetic engineering, allowing researchers to alter the genomes of a large range of organisms with relative ease. Experimental approaches based on this versatile technology have the potential to transform the field of cancer genetics. Here, we review current approaches for functional studies of cancer genes that are based on CRISPR-Cas, with emphasis on their applicability for the development of next-generation models of human cancer.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/NRC3950
