Detection of single-molecule H₂O₂ signalling from epidermal growth factor receptor using fluorescent single-walled carbon nanotubes
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Strano_Detection single-molecule.pdf
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Author(s)
Jin, Hong
Heller, Daniel A.
Kalbacova, Marie
Kim, Jong-Ho
Zhang, Jingqing
Boghossian, Ardemis A.
Maheshri, Narendra
Strano, Michael S.
Date Issued
March 2010
Journal
Nature Nanotechnology
Publisher
Nature Publishing Group
Citation
Jin, Hong, Daniel A. Heller, Marie Kalbacova, Jong-Ho Kim, Jingqing Zhang, Ardemis A. Boghossian, Narendra Maheshri, and Michael S. Strano. Detection of Single-molecule H₂O₂ Signalling from Epidermal Growth Factor Receptor Using Fluorescent Single-walled Carbon Nanotubes. Nature Nanotechnology 5, no. 4 (March 7, 2010): 302-309.
Version
Original manuscript
Abstract
An emerging concept in cell signalling is the natural role of reactive oxygen species such as hydrogen peroxide (H₂O₂) as beneficial messengers in redox signalling pathways. The nature of H₂O₂ signalling is confounded, however, by difficulties in tracking it in living systems, both spatially and temporally, at low concentrations. Here, we develop an array of fluorescent single-walled carbon nanotubes that can selectively record, in real time, the discrete, stochastic quenching events that occur as H₂O₂molecules are emitted from individual human epidermal carcinoma cells stimulated by epidermal growth factor. We show mathematically that such arrays can distinguish between molecules originating locally on the cell membrane from other contributions. We find that epidermal growth factor induces 2 nmol H₂O₂ locally over a period of 50 min. This platform promises a new approach to understanding the signalling of reactive oxygen species at the cellular level.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
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DOI of Published Version
https://doi.org/10.1038/nnano.2010.24
