Triacylglycerol Production from Corn Stover Using a Xylose-Fermenting Rhodococcus opacus Strain for Lignocellulosic Biofuels
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Sinskey_triacylglycerol-production.pdf
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1.33 MB
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Author(s)
Kurosawa, Kazuhiko
Wewetzer, Sandra J.
Sinskey, Anthony J
Date Issued
June 2014
Journal
Journal of Microbial & Biochemical Technology
Publisher
OMICS Publishing Group
Citation
Kurosawa, Kazuhiko, Sandra J. Wewetzer, and Anthony J. Sinskey. “Triacylglycerol Production from Corn Stover Using a Xylose-Fermenting Rhodococcus Opacus Strain for Lignocellulosic Biofuels.” Journal of Microbial & Biochemical Technology 06, no. 05 (2014).
Version
Final published version
Abstract
Triacylglycerols (TAGs) are in the spotlight as a feasible source of hydrocarbon-based biofuels. Rhodococcus opacus PD630 produces large amounts of intracellular TAGs in cultivations containing high concentrations of glucose, but it does not utilize xylose present in all hydrolysates of lignocellulosic biomass. We constructed a highpotency xylose-fermenting R. opacus strain MITXM-61 that exhibited robust growth and TAG biosynthesis on high concentrations of xylose by activating potential xylose-metabolism genes. MITXM-61 had the uncommon capacity to grow in defined media supplemented with xylose at concentrations of greater than 200 gl-1. MITXM-61 grown in corn stover hydrolysates containing 118 gl-1 of initial total sugars was capable of completely and simultaneously utilizing both xylose and glucose in the genuine lignocellulosic feedstock, and yielded 15.9 gl-1 of TAGs, corresponding to 54% of the cell dry weight. The oleaginous bacterium R. opacus strain proved useful for developing a new manufacturing paradigm to generate advanced lignocellulosic biofuels.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Engineering Systems Division
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DOI of Published Version
https://doi.org/10.4172/1948-5948.1000153
