Single-cell and metagenomic analyses indicate a fermentative and saccharolytic lifestyle for members of the OP9 lineage
Name
Ross_Single-cell.pdf
Size
1.93 MB
Format
Adobe PDF
Checksum (MD5)
e72f8b5d882499abc8287ea4589e4cd9
Author(s)
Dodsworth, Jeremy A.
Blainey, Paul C.
Murugapiran, Senthil K.
Swingley, Wesley D.
Ross, Christian A.
Tringe, Susannah G.
Chain, Patrick S. G.
Scholz, Matthew B.
Lo, Chien-Chi
Raymond, Jason
Date Issued
May 2013
Journal
Nature Communications
Publisher
Nature Publishing Group
Citation
Dodsworth, Jeremy A., Paul C. Blainey, Senthil K. Murugapiran, Wesley D. Swingley, Christian A. Ross, Susannah G. Tringe, Patrick S. G. Chain, et al. “Single-Cell and Metagenomic Analyses Indicate a Fermentative and Saccharolytic Lifestyle for Members of the OP9 Lineage.” Nat Comms 4 (May 14, 2013): 1854.
Version
Author's final manuscript
Abstract
OP9 is a yet-uncultivated bacterial lineage found in geothermal systems, petroleum reservoirs, anaerobic digesters and wastewater treatment facilities. Here we use single-cell and metagenome sequencing to obtain two distinct, nearly complete OP9 genomes, one constructed from single cells sorted from hot spring sediments and the other derived from binned metagenomic contigs from an in situ-enriched cellulolytic, thermophilic community. Phylogenomic analyses support the designation of OP9 as a candidate phylum for which we propose the name ‘Atribacteria’. Although a plurality of predicted proteins is most similar to those from Firmicutes, the presence of key genes suggests a diderm cell envelope. Metabolic reconstruction from the core genome suggests an anaerobic lifestyle based on sugar fermentation by Embden–Meyerhof glycolysis with production of hydrogen, acetate and ethanol. Putative glycohydrolases and an endoglucanase may enable catabolism of (hemi)cellulose in thermal environments. This study lays a foundation for understanding the physiology and ecological role of the ‘Atribacteria’.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/ncomms2884
