Resolving cell state in iPSC-derived human neural samples with multiplexed fluorescence imaging
Name
s42003-021-02276-x.pdf
Description
Published version
Size
4.55 MB
Format
Unknown
Checksum (MD5)
905733bc59d2f7a50263d25af430d910
Author(s)
Tomov, Martin L
O’Neil, Alison
Abbasi, Hamdah S
Cimini, Beth A
Carpenter, Anne E
Rubin, Lee L
Bathe, Mark
Date Issued
June 2021
Journal
Communications Biology
Publisher
Springer Science and Business Media LLC
Citation
Tomov, Martin L, O’Neil, Alison, Abbasi, Hamdah S, Cimini, Beth A, Carpenter, Anne E et al. 2021. "Resolving cell state in iPSC-derived human neural samples with multiplexed fluorescence imaging." Communications Biology, 4 (1).
Version
Final published version
Abstract
AbstractHuman induced pluripotent stem cell-derived (iPSC) neural cultures offer clinically relevant models of human diseases, including Amyotrophic Lateral Sclerosis, Alzheimer’s, and Autism Spectrum Disorder. In situ characterization of the spatial-temporal evolution of cell state in 3D culture and subsequent 2D dissociated culture models based on protein expression levels and localizations is essential to understanding neural cell differentiation, disease state phenotypes, and sample-to-sample variability. Here, we apply PRobe-based Imaging for Sequential Multiplexing (PRISM) to facilitate multiplexed imaging with facile, rapid exchange of imaging probes to analyze iPSC-derived cortical and motor neuron cultures that are relevant to psychiatric and neurodegenerative disease models, using over ten protein targets. Our approach permits analysis of cell differentiation, cell composition, and functional marker expression in complex stem-cell derived neural cultures. Furthermore, our approach is amenable to automation, offering in principle the ability to scale-up to dozens of protein targets and samples.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Terms of Use
Creative Commons Attribution 4.0 International license
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/s42003-021-02276-x
