Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(HB-co-HHx)) from butyrate using engineered Ralstonia eutropha
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Author(s)
Jeon, Jong-Min
Kim, Yong-Hyun
Kim, Hyun-Joong
Yi, Da-Hye
Kim, Hyungsup
Rha, ChoKyun
Yang, Yung-Hun
Brigham, Christopher J.
Sinskey, Anthony J
Date Issued
March 2014
Journal
Applied Microbiology and Biotechnology
Publisher
Springer Berlin Heidelberg
Citation
Jeon, Jong-Min et al. “Biosynthesis of poly(3-Hydroxybutyrate-Co-3-Hydroxyhexanoate) (P(HB-Co-HHx)) from Butyrate Using Engineered Ralstonia Eutropha.” Applied Microbiology and Biotechnology 98.12 (2014): 5461–5469.
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Author's final manuscript
Abstract
Polyhydroxyalkanoates (PHAs), a promising family of bio-based polymers, are considered to be alternatives to traditional petroleum-based plastics. Copolymers like poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(HB-co-HHx)) have been shown to exhibit favorable physical and mechanical properties, due to decreased crystallinity resulting from the presence of medium-chain-length 3-hydroxyhexanoate (3HHx) monomers. In this study, we produced P(HB-co-HHx) using engineered Ralstonia eutropha strains containing deletions of the acetoacetyl-CoA reductase (phaB) genes and replacing the native PHA synthase with phaC2 from Rhodococcus aetherivorans I24 and by using butyrate, a short-chain organic acid, as the carbon source. Although the wild-type R. eutropha did not produce P(HB-co-HHx) when grown on mixed acids or on butyrate as the sole carbon source, we are able to produce polymer containing up to 40 wt% 3HHx monomer with the aforementioned engineered R. eutropha strains using various concentrations of just butyrate as the sole carbon source. This is the first report for the production of P(HB-co-HHx) copolymer in R. eutropha using butyrate.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Engineering Systems Division
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DOI of Published Version
https://doi.org/10.1007/s00253-014-5617-7
