Integrated Microfluidic Culture Media Oxygenator for Organ-on-a-Chip Applications
Name
Dey Barsukova-anitadb1-sb-meche-2022-thesis.pdf APPROVED.pdf
Description
Thesis PDF
Size
15 MB
Format
Adobe PDF
Checksum (MD5)
4c9cd442f3ca9a4f037edd6ec53fcccd
Author(s)
Dey Barsukova, Anita
Advisor(s)
David L. Trumper
Date Issued
May 2022
Publisher
Massachusetts Institute of Technology
Abstract
Microphysiological systems (MPS) are in vitro platforms for the culture of human cells in a manner that closely mimics the in vivo physiological microenvironment. Oxygen is a key element for maintaining cell viability and function in MPS devices. Thus, this thesis presents the design, fabrication, and testing of a microfluidic oxygenator chip, or "Oxychip", for providing control of oxygen concentration in MPS cell culture media. The oxygenation mechanism of the device features a serpentine fluidic channel that contacts a pneumatic pocket through a gas permeable membrane, to allow for oxygen exchange between the liquid media and the gas in the pneumatic compartment. Additionally, the Oxychip integrates the oxygenator module with an on-board micropump, pressure regulator, culture media reservoir, and oxygen probe interface, in a compact 40 x 25 mm microfluidic chip. To validate the functionality of the device, a series of three experiments were conducted in which oxygen partial pressure within circulating fluid media was monitored using oxygen probes, and rapid deoxygenation and re-oxygenation of fluid media was demonstrated.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
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