Video-rate tomographic phase microscopy
Name
Fang-Yen-2011-Video-rate tomographic phase microscopy.pdf
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Author(s)
Fang-Yen, Chris
Choi, Wonshik
Sung, Yongjin
Holbrow, Charles J.
Dasari, Ramachandra Rao
Feld, Michael S.
Date Issued
January 2011
Journal
Journal of Biomedical Optics
Publisher
SPIE
Citation
Christopher Fang-Yen, Wonshik Choi, Yongjin Sung, Charles J. Holbrow, Ramachandra R. Dasari and Michael S. Feld, "Video-rate tomographic phase microscopy", J. Biomed. Opt. 16, 011005 (Jan 14, 2011); doi:10.1117/1.3522506 © 2011 Society of Photo-Optical Instrumentation Engineers
Version
Final published version
Abstract
Tomographic phase microscopy measures the 3-D refractive index distribution of cells and tissues
by combining the information from a series of angle-dependent interferometric phase images. In the original
device, the frame rate was limited to 0.1 frames per second (fps) by the technique used to acquire phase images,
preventing measurements of moving or rapidly changing samples. We describe an improved tomographic phase
microscope in which phase images are acquired via a spatial fringe pattern demodulation method, enabling a full
tomogram acquisition rate of 30 fps. In addition, in this system the refractive index is calculated by a diffraction
tomography algorithm that accounts for the effects of diffraction in the 3-D reconstruction.We use the instrument to
quantitatively monitor rapid changes in refractive index within defined subregions of cells due to exposure to acetic
acid or changes in medium osmolarity.
MIT Department
Massachusetts Institute of Technology. Spectroscopy Laboratory
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1117/1.3522506
