miMeasure – a standard for miRNA binding site characterization in mammalian cells
Name
BBFRFC73.pdf
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375.58 KB
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Author(s)
Adlung, Lorenz
Sabah, Jude Al
Bayer, Philipp
Berrens, Rebecca
Cristiano, Elena
Flocke, Lea
Kleinsorg, Stefan
Kolodziejczyk, Aleksandra
Kraemer, Stephen
Torre, Alejandro Macias
Date Issued
December 5, 2010
Series/Report no.
BBF RFC;73
Abstract
This RFC proposes a standard for the quantitative characterization of miRNA binding sites (miRNA-BS) in mammalian cells. The miMeasure standard introduces a ready-to-use standard measurement plasmid (pSMB_miMeasure, BBa_K337049) enabling rapid experimental characterization of any miRNA-BS of choice. We recommend a new standard unit, RKDU (relative knock-down unit) to describe the knock-down efficiency of a miRNA-BS in a specific cell type. pSMB_miMeasure allows for an easy and fast measurement of RKDU while providing effective normalization against variance stemming from differences in transfection efficiency and from other sources.
Subjects
measurement standard
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Attribution-No Derivative Works 3.0 United States
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