Quantitative Detection of Active Vibrios Associated with White Plague Disease in Mussismilia braziliensis Corals
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fmicb-08-02272.pdf
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1.12 MB
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Checksum (MD5)
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Author(s)
Moreira, Ana P. B.
Garcia, Gizele D.
Berlinck, Roberto G. S.
Thompson, Cristiane C.
Thompson, Fabiano L.
Alessandra Chimetto Tonon, Luciane
Thompson, Janelle Renee
Penn, Kevin
Lim, Rachelle A.
Date Issued
November 2017
Journal
Frontiers in Microbiology
Publisher
Frontiers Research Foundation
Citation
Chimetto Tonon, Luciane A. et al. “Quantitative Detection of Active Vibrios Associated with White Plague Disease in Mussismilia Braziliensis Corals.” Frontiers in Microbiology 8 (November 2017): 2272 © 2017 Chimetto Tonon et al.
Version
Final published version
Abstract
Over recent decades several coral diseases have been reported as a significant threat to coral reef ecosystems causing the decline of corals cover and diversity around the world. The development of techniques that improve the ability to detect and quantify microbial agents involved in coral disease will aid in the elucidation of disease cause, facilitating coral disease detection and diagnosis, identification and pathogen monitoring, pathogen sources, vectors, and reservoirs. The genus Vibrio is known to harbor pathogenic strains to marine organisms. One of the best-characterized coral pathogens is Vibrio coralliilyticus, an aetilogic agent of White Plague Disease (WPD). We used Mussismilia coral tissue (healthy and diseased specimens) to develop a rapid reproducible detection system for vibrios based on RT-QPCR and SYBR chemistry. We were able to detect total vibrios in expressed RNA targeting the 16S rRNA gene at 5.23 × 10 6 copies/μg RNA and V. coralliilyticus targeting the pyrH gene at 5.10 × 10 3 copies/μg RNA in coral tissue. Detection of V. coralliilyticus in diseased and in healthy samples suggests that WPD in the Abrolhos Bank may be caused by a consortium of microorganism and not only a single pathogen. We developed a more practical and economic system compared with probe uses for the real-time detection and quantif ication of vibrios from coral tissues by using the 16S rRNA and pyrH gene. This qPCR assay is a reliable tool for the monitoring of coral pathogens, and can be useful to prevent, control, or reduce impacts in this ecosystem.
MIT Department
Massachusetts Institute of Technology. Department of Civil and Environmental Engineering
Terms of Use
Attribution 4.0 International (CC BY 4.0)
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DOI of Published Version
https://doi.org/10.3389/fmicb.2017.02272
